NGS_raw_data
Description
To investigate which of the intrinsic of a horizontally acquired gene and the environment can be a barrier to HGT, we transferred and expressed 44 E.coli orthologs into S. Typhimurium strain 4/74. Pooled growth assays were performed for the 44 mutants and a WT in four infection relevant environments that are representative of an intra-macrophage environment during an infection. We estimated relative fitness effects in four infection relevant environments for the 44 Salmonella mutants with changes in gene frequencies estimated using Next-Generation Sequencing. Preprint can be found at https://www.biorxiv.org/content/10.1101/2022.04.01.486712v2.full Codes for processing the reads and all analysis carried out in the paper are available on GitHub. https://github.com/ramabht/Barriers_to_HGT