NGS_raw_data

Published: 17 January 2023| Version 1 | DOI: 10.17632/44dtg2m6y9.1
Contributors:
Rama Bhatia,
,
,
,

Description

To investigate which of the intrinsic of a horizontally acquired gene and the environment can be a barrier to HGT, we transferred and expressed 44 E.coli orthologs into S. Typhimurium strain 4/74. Pooled growth assays were performed for the 44 mutants and a WT in four infection relevant environments that are representative of an intra-macrophage environment during an infection. We estimated relative fitness effects in four infection relevant environments for the 44 Salmonella mutants with changes in gene frequencies estimated using Next-Generation Sequencing. Preprint can be found at https://www.biorxiv.org/content/10.1101/2022.04.01.486712v2.full Codes for processing the reads and all analysis carried out in the paper are available on GitHub. https://github.com/ramabht/Barriers_to_HGT

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Institutions

University of Liverpool, University of Oklahoma

Categories

Microbial Evolution, Bacterial Genetics, Salmonella

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