Supplemental material for Fructo-oligosaccharides supplementation enhances the growth of nursing dairy calves while stimulating the persistence of Bifidobacterium and hindgut microbiome’s maturation

Published: 16 May 2024| Version 1 | DOI: 10.17632/44ywnhg89m.1
yunlong gao


Supplemental Figure S1. Impact of FOS supplementation on microbial community structure. (A) Bar plot displaying microbial composition at the phylum level. Microbial taxa with an average relative abundance < 3% were grouped into "Others." (B) Bar plot illustrating microbial composition at the phylum level. Microbial taxa with an average relative abundance < 0.5% were grouped into "Others." (C) Discriminative microbiotas at the genus level between the CON group and FOS group were identified through linear discriminant analysis effect size (LEfSe). (D) Bacterial biomarkers distinguishing FOS supplementation were determined using a random forest classification model with ten-fold cross-validation for ten replicates. (E) The distribution of sequencing read quality is depicted, showcasing the transition from raw data to processed data after quality control. "Raw Data" refers to the initial dataset, while "Processed Data" represents the clean data obtained post-quality control. Supplemental Figure S2. Microbiome community type assessment in the bacterial microbiome of preterm infants. (A) Microbiome community types were delineated through hierarchical clustering by cutting the tree into 7 clusters. (B) Bacterial heterogeneity was compared using the inverse of the coefficient of variation (ICV) across community types. (C) A heatmap visualizes the most abundant taxa at the genus level based on the intervention and community type (cluster). (D) Time-to-event analysis illustrates that FOS expedites the transition into the C7 mature community type, as indicated by the Kaplan-Meier curve depicting the probability of not reaching the mature community type. Supplemental Figure S3. The dynamic distribution of FOS-degrading enzyme (GH68) in the two groups.



Nanjing Agricultural University