Original Western blot and DNA gel electrophoresis images - Sarkar et al

Published: 12 January 2022| Version 1 | DOI: 10.17632/46dvzxsbzs.1
Contributor:
Rahul Pal

Description

The post-menopausal state in women is associated with increased cancer incidence, the reasons for which remain obscure. Curiously, increased circulating levels of beta-hCG (a hormonal subunit linked with tumors of several lineages), are also often observed post-menopause. This study reveals that the transgenic expression of beta-hCG in mice, particularly in the absence of the ovaries (a mimic of the post-menopausal state), constitutes a significant pro-tumorigenic signal; further, administration of progesterone has significant anti-tumor effects. Progesterone mediates apoptosis in tumor cells, acting via non-nuclear receptors, subsequent to phosphorylation of p38. RNA-seq profiling identified molecular signatures associated with these processes. qPCR and IHC provided validation. TCGA analysis revealed several human correlates (including expression of intra-tumoral beta-hCG) between post-menopausal status and poorer prognosis in patients of lung adenocarcinoma, colon adenocarcinoma and glioblastoma. Treatment of post-menopausal cancer patients with progesterone, particularly upon the detection of circulating beta-hCG (either intra- or extra-tumoral), may prove beneficial. The data presented here shows original Western blot and DNA gel electrophoresis images for the manuscript by Sarkar et al., placed here as supporting evidence. The agarose gels are aimed at detecting the presence of various forms of the progesterone receptor in LLC1 tumor cells grown in vitro as well as in LLC1 tumors excised from mice. Also included is data on the expression of the beta-hCG transgene in mice, and the experiments to detect the presence of the the conventional LH/CG receptor on tumor cells. The Western blots depict experiments carried out to determine the signaling pathways progesterone employs in tumor cells to mediate its effects. Blots depicting the various caspases cleaved upon progesterone action in tumor cell are also shown. Whether hCG or beta-hCG could prevent the progesterone-induced cleavage of caspase 3 was also assessed.

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National Institute of Immunology

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Animal Model of Tumor

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