Hsa-miR-1587 binding proteins: Human proteome microarray dataset

Published: 21 May 2020| Version 1 | DOI: 10.17632/4ghzsy8m35.1
Contributors:
Lulu Zhang,
Jiang Zhou,
Ming Xu,
Gu Yuan

Description

Table 1. The scanning data of proteome microarray after incubation with miR-1587 G-quadruplex. The miR-1587 was labelled with FAM and then dissolved in 30 mM Tris-HCl / 150 mM KCl to form G-quadruplex, followed by incubation with the microarray. The microarray was then scanned at 488 nm using a GenePix 4200A microarray scanner (Axon Instruments, Molecular Devices, CA, USA) to visualize. GenePix Pro-6.0 software (Axon Instruments, Molecular Devices, CA, USA) was used for data reading. Table 2. The scanning data of proteome microarray after incubation with free form miR-1587. The miR-1587 was labelled with FAM and then dissolved in 30 mM Tris-HCl and had a free form in solution. After incubation with the free form miR-1587, the microarray was scanned at 488 nm using a GenePix 4200A microarray scanner (Axon Instruments, Molecular Devices, CA, USA) to visualize. GenePix Pro-6.0 software (Axon Instruments, Molecular Devices, CA, USA) was used for data reading.

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Molecular Biology, MicroRNA, Microarray

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