6S RNA mimics B-form DNA to regulate Escherichia coli RNA polymerase (MOLECULAR-CELL-D-17-01031). Chen, et al.
Description
Files contain raw unprocessed data used in Chen et al. "6S RNA mimics B-form DNA to regulate Escherichia coli RNA polymerase". Please see paper for figure labels and legends as well as details on how experiments were performed. Figure S1 -‘5labeled_footprinting’ and ‘3labeled_footprinting’ gel images are labeled in Figure S1B -Lanes 1 and 2 of ‘transcription_assay_6S_deltaCS’ correspond to Figure S1C (left panel) -Lanes 5 and 6 of ‘transcription_assay_6S_RNA_mutant’ correspond to Figure S1C (right panel) Figure S5 -‘XL_test_copy’ is labeled in Figure S5A -Boxed part of ‘gel3_XLhot_copy’ is represented and labeled in Figure S5B -Boxed part of ‘PE_mapping_copy’ is represented and labeled in Figure S5C Figure S6 -Boxed part of ‘stem_mutants_copy’ is represented and labeled in Figure S6A -Boxed part of ‘G82_mutants_binding copy’ is represented and labeled in Figure S6B -Boxed part of ‘binding_131_135_136_others_copy’ is represented and labeled in Figure S6C -Lanes 1-12 of ‘Wt_FYWW_T7A1DS_T7A1bubble’ correspond to Figure S6E (top panel) -Lanes 13-24 of ‘Wt_FYWW_T7A1DS_T7A1bubble’ correspond to Figure S6E (middle panel) -‘Wt_FYWW_6S_deltaCS’ is represented in Figure S6E (bottom panel) Figure 7 Gels used to generate Figure 7C are oriented to have the following loading ordering (left to right): 1) T7A1 – Wt SigS 2) T7A1 – SigS E308K/E315H 3) T7A1 – Wt Sig70 4) T7A1 – Sig70 K593E/H600E 5) DPS100 – Wt SigS 6) DPS100 – SigS E308K/E315H 7) DPS100 – Wt Sig70 8) DPS100 – Sig70 K593E/H600E 9) 6S RNA – Wt SigS 10) 6S RNA – SigS E308K/E315H 11) 6S RNA – Wt Sig70 12) 6S RNA – Sig70 K593E/H600E *File names also denote the trial number. Figure S7 Gels used to generate Figure S7B are oriented to have the following loading ordering (left to right): 1) Wt SigS 2) SigS L116F 3) SigS E217K 4) SigS G228D 5) SigS E308K 6) SigS E315H 7) Wt Sig70 8) Sig70 F401L 9) Sig70 K502E 10) Sig70 D513G 11) Sig70 K593E 12) Sig70 H600E *File names also denote transcription template used and trial number.