Protection of the endothelium and endothelial glycocalyx by hydrogen against ischemia-reperfusion injury in a porcine model of cardiac arrest

Published: 12 December 2022| Version 1 | DOI: 10.17632/4km8rgwwpk.1
Contributor:
David Astapenko

Description

Hydrogen is a potent antioxidant agent that can easily be administered by inhalation. The aim of the study was to evaluate whether hydrogen protects the glycocalyx layer after successful cardiopulmonary resuscitation (CPR). Fourteen anesthetized piglets underwent CPR after induced ventricular fibrillation. During CPR and return of spontaneous circulation, 2% hydrogen gas was administered to seven pigs (hydrogen group) and seven constituted a control group. Biochemistry and sublingual microcirculation were assessed at baseline, during CPR, at the 15th, 30th, 60th, and 120th minute. All seven subjects from the hydrogen group and six subjects in the control group were resuscitated after 6–10 minutes. At baseline, there were no statistically significant differences in examined variables. After the CPR, blood pH, base excess, and lactate showed significantly smaller derangement in the hydrogen group than in the control group. By contrast, plasma syndecan-1 and the variables in sublingual microcirculation did not change after the CPR; here, the measured values were virtually identical between the groups. In pigs, hydrogen gas inhalation during CPR and post-resuscitation care was associated with less pronounced metabolic acidosis than in controls. However, we could not find evidence of injury to the endothelium or glycocalyx in any studied groups.

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Hydrogen is a potent antioxidant agent that can easily be administered by inhalation. The aim of the study was to evaluate whether hydrogen protects the glycocalyx layer after successful cardiopulmonary resuscitation (CPR). Fourteen anesthetized piglets underwent CPR after induced ventricular fibrillation. During CPR and return of spontaneous circulation, 2% hydrogen gas was administered to seven pigs (hydrogen group) and seven constituted a control group. Biochemistry and sublingual microcirculation were assessed at baseline, during CPR, at the 15th, 30th, 60th, and 120th minute. All seven subjects from the hydrogen group and six subjects in the control group were resuscitated after 6–10 minutes. At baseline, there were no statistically significant differences in examined variables. After the CPR, blood pH, base excess, and lactate showed significantly smaller derangement in the hydrogen group than in the control group. By contrast, plasma syndecan-1 and the variables in sublingual microcirculation did not change after the CPR; here, the measured values were virtually identical between the groups. In pigs, hydrogen gas inhalation during CPR and post-resuscitation care was associated with less pronounced metabolic acidosis than in controls. However, we could not find evidence of injury to the endothelium or glycocalyx in any studied groups.

Institutions

Fakultni Nemocnice Hradec Kralove, Univerzita Karlova Lekarska fakulta v Hradci Kralove

Categories

Reperfusion Injury, Antioxidant, Cardiopulmonary Resuscitation

Funding

Fakultní nemocnice Hradec Králové

MH CZ-DRO (UHHK, 00179906)

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