A comprehensive Drosophila resource to identify key functional interactions between SARS-CoV-2 factors and host proteins, Guichard, Lu, Kanca et al.
Description
Table S1. List of the UAS-SARS-CoV-2 transgenic flies. The docking site and BDSC stock number of each fly line are provided. UAS-NSP lines including a KOZAK sequence to increase expression levels do not include an immunological tag. Table S2. Available UAS-human cDNA stocks encoding SARS-CoV-2 protein interactors. Stock numbers for Bloomington and Kyoto Drosophila stock centers are indicated. To obtain stocks from Kyoto, an account must be opened at the KDSC (https://kyotofly.kit.jp/cgi-bin/stocks/index.cgi). After placing an order, a letter of No Permit Required (LONPR) must be provided, which can be obtained by requesting at USDA (brsnopermitrequired@usda.gov) and attaching a filled application, which can be found at: https://bdsc.indiana.edu/pdf/USDA/LONPR_form.pdf. For lines that are not available yet, UAS clones are or will soon be available through the DGRC (searchable through https://dgrc.bio.indiana.edu/clones/Catalog under “Human ORF”). Current available clones can be found in https://dgrc.bio.indiana.edu/include/file/DGRC-HumanORF-clone-list-2023-05-05.xlsx. Table S3. Available T2A-GAL4 or Kozak-GAL4 for fly ortholog candidates that encode for SARS-CoV-2 human interactors. For lines that have not been assigned a BDSC stock number at the time of publication, the CRIMIC number can be entered in the BDSC search bar to locate the stock number and line as it becomes available. Table S4. Summary of lethality phenotypes induced by SARS-CoV-2 factors driven by DaGAL4 at 29oC, indicating the developmental timing of lethality. Table S5. List of human interactors identified in five interactome studies 9 7,11 12 10, showing which interactors have been identified again in subsequent studies. Candidates identified in the 9 study are outlined in yellow.