Dataset on characterisation and stability of gingival retraction cord lignocaine-adrenaline nanogel optimised using central composite design

Description

The compatibility study on the pharmaceutical ingredients; lignocaine and adrenaline with the excipients was done using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) and ultraviolet-visible (UV-Vis) spectroscopy were used for quantitative analysis of the mixtures. The optimisation of the lignocaine-adrenaline nanoemulsion (LANE) formulation was analysed using Design-Expert software version 11, a randomised Central Composite Design (CCD) Response Surface Methodology (RSM) where it computes the effects of the independent factors and was used around a fixed point for the LANE preparations. This research data encompasses the dental aspects of restorative treatment with focus on gingival retraction cords usage in gingival management division. This research was funded by The Ministry of Higher Education of Malaysia; Fundamental Research Grant Scheme (FRGS/1/2022/SKK11/UIAM/03/3). The data and supplementary material are divided in 2 files: The accuracy study of LANE at one-point concentration where The LANE suspension was tested with triplicate examination using Uv-Vis spectrophotometer. The result of percentage recovery of lignocaine and adrenaline for LANE were in acceptable level of accuracy which is from 98%-102% mean recovery of drug substances in accordance with United States Pharmacopeia (USP)-857 guideline. The CCD dataset contains the independent (Xi) and dependent (Yi) variables used in CCD with levels and desirability constraint. Three independent variables (Xi) were considered in this study: Triacetin (w/w) % (X1), polysorbate 80 (Tween80) (w/w) % (X2), and polyethylene glycol 400 (PEG 400) (w/w) % (X3) where it displays the range chosen for each independent variable. Z-average (nm) (Y1), PDI (Y2), zeta potential (mV) (Y3), and pH (Y4) were the dependent variables (Yi) that were taken into consideration and the displays of the intended constraints for every dependent variable. The experiments were designed using 3 factors with 4 central point generating 18 runs as shown in dataset CCD system composition. Each experiment was conducted independently following the runs formulated from the CCD output accordingly. CCD then will produce prediction of optimised LANE formulation based on the Yi analysis and the comparison with the actual test of the selected LANE. Taking into consideration the lowest surfactant concentration possible, the variety of NE constitutions were derived from the calculation of the CCD and for those that did not exhibit drug precipitation or phase separation during preparation or after 72 hours of storage at 25°C were selected for nano-based gel (NBG). Following the constraint applied on the response variables in CCD optimisation criteria, the CCD gave out the numerical solutions of the independent variables with highest desirability of the triacetin, tween 80, and PEG400 at 5%, 14%, and 8% respectively. The CCD point prediction and the actual result of the optimised LANE all done in triplicates.

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