Chatterjee et al. AhR Western blot Cell Reports 2023

Published: 16 May 2023| Version 1 | DOI: 10.17632/4ry9gxv7tp.1
Petronela Ancuta,


These figures depict western blot results for AhR and b actin protein visualisation and quantification upon CRISPR/Cas9 gene editing and/or TCR triggering: Figure 1B, Figure 1C, Supplemental Figure 1A and Supplemental Figure 2C.


Steps to reproduce

Western Blot Method Cells were lysed with the RIPA buffer (Cell Signaling) supplemented with phosphatase inhibitors (PhosSTOP) and protease inhibitor cocktail (Complete Mini EDTA-free, Roche) for 10 minutes at 4 °C and centrifuged at 14,000 g for 10 minutes. Proteins were quantified by Bradford assay (Bio-Rad, Hercules, CA, USA). Samples (15-30 µg protein/well) were loaded on SDS PAGE gel (90 minutes, 100 V), transferred to PVDF membrane (Millipore) and blocked with 5% BSA. Subsequently membranes were incubated with primary AhR antibodies (Clone ab190797, Abcam, Cambridge, UK) and β-actin (Sigma, USA). Immunoreactive bands were detected using HRP-conjugated secondary antibodies and revealed with ECL substrate (GE Healthcare, Chicago, IL, USA) (Supplemental Tables 2; Key Resources Table). See manuscript by Chatterjee D et al., accepted in Cell Reports, 2023.


Universite de Montreal


Western Blot


Canadian Institutes of Health Research


Canadian Institutes of Health Research


Canadian Institutes of Health Research

PJT #153052; PJT 178127