MatLab program for precision calibration of optical tweezers

Published: 1 June 2004| Version 1 | DOI: 10.17632/4vjf735s6h.1
Iva Marija Tolić-Nørrelykke, Kirstine Berg-Sørensen, Henrik Flyvbjerg


Abstract Optical tweezers are used as force transducers in many types of experiments. The force they exert in a given experiment is known only after a calibration. Computer codes that calibrate optical tweezers with high precision and reliability in the (x,y)-plane orthogonal to the laser beam axis were written in MatLab (MathWorks Inc.) and are presented here. The calibration is based on the power spectrum of the Brownian motion of a dielectric bead trapped in the tweezers. Precision is achieved by a... Title of program: tweezercalib Catalogue Id: ADTV_v1_0 Nature of problem To calibrate optical tweezers with precision by fitting theory to experimental power spectrum of position of bead doing Brownian motion in incompressible fluid, possibly near microscope cover slip, while trapped in optical tweezers. Thereby determine spring constant of optical trap and conversion factor for arbitrary-units-to-nanometers for detection system. Versions of this program held in the CPC repository in Mendeley Data ADTV_v1_0; tweezercalib; 10.1016/j.cpc.2004.02.012 ADTV_v2_0; tweezercalib; 10.1016/j.cpc.2005.11.007 ADTV_v2_1; tweezercalib; 10.1016/j.cpc.2006.07.009 This program has been imported from the CPC Program Library held at Queen's University Belfast (1969-2018)



Optics, Molecular Biology, Statistical Physics, Biological Sciences, Computational Physics, Thermodynamics, Computational Method