Software/Code for: CaV2.1 α1 subunit expression regulates presynaptic CaV2.1 abundance and synaptic strength at a central synapse

Published: 26 November 2018| Version 1 | DOI: 10.17632/4ycrjtpfc6.1
Contributors:
Matthias Luebbert, Rene Oliver Goral, Christian Keine, Connan Thomas, Debbie Guerrero-Given, Travis Putzke, Rachel Satterfield, Naomi Kamasawa, Samuel M. Young, Jr.

Description

contains custom-written functions used in "CaV2.1 α1 subunit expression regulates presynaptic CaV2.1 abundance and synaptic strength at a central synapse".

Files

Steps to reproduce

-calculates synaptic parameters by fitting synaptic currents from train stimulation to NpRf model. Estimates N (RRP size), p (initial release probability), R (replenishment), and f (synaptic facilitation). This model was developed and described in "Thanawala MS, Regehr WG (2016) Determining Synaptic Parameters Using High-Frequency Activation. J Neurosci Methods 264:136–152." (http://dx.doi.org/10.1016/j.jneumeth.2016.02.021); How to use: Input: - EPSC/IPSC amplitudes from train stimulation, units are not important, but the estimated RRP will be in the same units Output: - Struct containing estimated values for N, p, R, and f To run call the function NpRfModel.m with an data vector containing EPSC/IPSC amplitudes during train stimulation. When using, please cite the original publication: "Thanawala MS, Regehr WG (2016) Determining Synaptic Parameters Using High-Frequency Activation. J Neurosci Methods 264:136–152." (http://dx.doi.org/10.1016/j.jneumeth.2016.02.021) - requires Optimization Toolbox and Curve Fitting Toolbox in Matlab (tm).

Institutions

University of Iowa, Max Planck Florida Institute, University of Iowa Roy J and Lucille A Carver College of Medicine

Categories

Software, Synaptic Vesicle Release, Synaptic Physiology, Calcium Channels, Calcium Channel Expression, Presynaptic Mechanisms of Synaptic Transmission

Licence