In vitro detoxification of aflatoxin B1 by aqueous extracts of medicinal herbs
Description
Detoxification of aflatoxin B1 by aqueous extracts of medicinal herbs
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One g of herbal powder was mixed with 5 ml of sterile distilled water (SDW) and allowed to stand overnight at 4°C. After incubation the mixture was centrifuged at 12,000 × g for 15 min at 4°C and the supernatant was collected. The volume of the extract was made up to 5 ml with SDW. AFB1 detoxification potential of herbal extracts was determined as described by Velazhahan et al. (2010) with some modifications. Briefly, 250 μl of plant extract in a 1.5 ml centrifuge tube was mixed with 50 μl of AFB1 (50 μg/L) and incubated at 37°C in a water bath. After 48 h, 250 μl of chloroform was added, vortexed and then centrifuged at 12,000 × g for 15 min. The chloroform layer was collected in a new tube and evaporated at 60°C in a water bath. The residue was suspended in 50 μl of 70% methanol and AFB1 content was determined by ELISA (RIDASCREEN Aflatoxin B1; R-Biopharm AG, Darmstadt, Germany) by following the manufacturer’s instructions. The degraded products of AFB1 after treatment with herbal extracts were analyzed by Agilent LC/MS/MS. Mass spectra data were acquired and processed by Agilent MassHunter workstation and Agilent MassHunter qualitative analysis software, respectively.