Assis et al., 2022 - Original Data set

Published: 23 August 2022| Version 1 | DOI: 10.17632/4zrvwrrxx7.1
Vania Regina Assis


This is the original raw data set (before transformations) used in the data analyses from the manuscript: "The Impacts of Transdermal Application of Corticosterone on Toad (Rhinella icterica) Immunity", published in Integrative and Comparative Biology.


Steps to reproduce

Descriptive statistics were performed for all variables. Shapiro-Wilk normality test and Levene's test for homogeneity of variance were used to evaluate data for ANCOVAs and ANOVAs assumptions; therefore, CORT, PP, and PE were log10 transformed. Unstandardized residuals of a linear regression of body mass as a function of SVL were used as body index (BI). Both body mass and BI were used as covariates on a set of ANCOVAs using treatment (vehicle, low (1ug), and high (10ug) dose) as factors and CORT, T, BKA, NLR, PP, and PE as dependent variables. When the covariates did not affect the variables, they were excluded from the model, and an ANOVA was performed instead. Following ANCOVAs and ANOVAs, simple independent contrasts were performed to compare CORT doses (1 and 10ug) with the vehicle group. Statistical analyses were run using IBM SPSS Statistics 26.


Universidade de Sao Paulo Editora da Universidade de Sao Paulo