Data on the inner filter effect, suspended solids and nitrate interferences in fluorescence measurements of wastewater organic matter

Published: 2 December 2019| Version 1 | DOI: 10.17632/4zss49jycj.1


Data in this repository contain raw fluorescence and absorbance measurements of dilution series of three wastewater effluents and a surface water located in Sicily (Italy). Identical dilution series were prepared for unfiltered samples, and for samples filtered by 0.7 μm glass microfiber filters (Whatman, Clifton - NJ). Dilution series were prepared by diluting the original sample with Milli-Q water in a final volume of 100 mL. The volume of water sample diluted in Milli-Q water was progressively reduced by a step of 5/2.5 mL to obtain the dilution series. A further dataset of Fluorescence and UV absorbance measurements is related to samples obtained adding different amount of nitrates in a wastewater effluent. Ultraviolet light absorbance was analyzed using a Shimadzu UV-1800 spectrophotometer (Kyoto, Japan). Absorbance spectra were measured from 200 to 800 nm at 1 nm intervals in a 1 cm quartz cuvette with Milli-Q water used as a blank. Fluorescence data were collected using a Shimadzu RF-5301PC fluorescence spectrophotometer (Kyoto, Japan) with the scanning range from excitation wavelength 220 nm to 450 nm at an interval of 5 nm, and emission wavelength from 250 nm to 580 nm at the interval of 1 nm. Excitation and emission slit widths were both set at 5 nm.



Universita degli Studi di Catania


Spectroscopy, Environmental Engineering, Fluorescence, Absorbance