PAR-2 gene expression in PDGF-BB stimulated rabbit Achilles tenocytes in vitro

Published: 7 July 2024| Version 1 | DOI: 10.17632/55fk93mssn.1
Contributor:
Johanna Buschmann

Description

Two data sets are presented: The first data set (“qPCR 4A_xlsx”) refers to PAR-2 gene expression in rabbit Achilles tenocyte culture under stimulation of PDGF-BB supplementation (with 20 ng/mL PDGF-BB or without supplementation of PDGF-BB) as a function of time; at days 3, 7 and 14 in vitro. The second data set (“qPCR 4B_xlsx”) represents PAR-2 gene expression in rabbit Achilles tenocytes on day 3 under stimulation of PDGF-BB supplementation (with 20 ng/mL PDGF-BB or without supplementation of PDGF-BB) in the presence or absence of inhibitors in a 40 μM concentration and 5 % fetal bovine serum in vitro. The following inhibitors were applied: SB203580 = inhibitor for MAPK; LY-294002 = inhibitor for PI3K; PD153035 = inhibitor for EGFR. Furthermore, gene expression was assessed under DMSO = dimethyl sulfoxide (solvent of the inhibitors) or in medium = basal culture medium (with 10 % fetal bovine serum).

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Steps to reproduce

Rabbit Achilles tenocyte cultures were stimulated with 20 ng/mL PDGF-BB and compared to the corresponding cultures without PDGF-BB supplementation. qPCR data for the PAR-2 gene (target gene rbF2rl1) expression were assessed. Several inhibitors were tested in stimulated and non-stimulated tenocyte cultures

Institutions

Universitat Zurich, UniversitatsSpital Zurich

Categories

Biological Database

Funding

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung

310030_197578

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