Phenotypic and genotypic analysis of benzimidazole resistance in reciprocal genetic crosses of Haemonchus contortus

Description

The sequence reads were classified as β-tubulin isotype 1, a count list of the consensus sequences of each sample was created using the ‘unique.seqs’ command. The count list was further used to create FASTQ files of the consensus sequences of each sample using the ‘split.abund’ command to sort data into groups of rare and abundant based on the cutoff value (1,000 reads ), followed by the ‘split.groups’ command. H. contortus β-tubulin isotype 1 isolates consensus sequences generated from the count list of the consensus sequences were edited and aligned in Geneious Prime 2020.1

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