Metagenomic Dataset of an Eastern spinebill (Acanthorhynchus tenuirostris)

Published: 06-04-2021| Version 2 | DOI: 10.17632/57zhhhs7xz.2
Contributor:
Subir Sarker

Description

2.1 Source of sampling and extraction of DNA Droppings was collected from a holding bag of an Eastern spinebill caught in a mist net as part of a fauna survey in the Windsor Downs Reserve Nature (33°39’0.42’S, 150°48’31.97”E), New South Wales (Australian Bird and Bat Banding Scheme (ABBBS) Banding Authority Number 1893, ABBBS project approval - cooperative project 8529, New South Wales National Parks and Wildlife Service - Scientific License No. SL101929). The total genomic DNA was extracted using a commercial kit (PurelinkTM Genomic DNA Mini Kit, Invitrogen, California, USA) according to the manufacturer’s instructions. 2.2 Library construction and sequencing The library preparation and sequencing was performed as previously described (Sarker et al., 2020; Sarker et al., 2018; Sarker et al., 2017b). Briefly, the paired-end library with an insert size of 150 bp was prepared using the QIAseq FX DNA Library Kit (Qiagen) starting with ten ng of total genomic DNA (gDNA). The amplified library was cleaned to remove PCR-generated adaptor-dimers using JetSeq™ Clean beads (Bioline) according to the protocol described in QIAseq FX DNA Library Kits. The quality and quantity of the prepared library was assessed using an Agilent Tape Station (Agilent Technologies) by the Genomic Platform, La Trobe University. The prepared library was normalised and pooled in equimolar quantities. Cluster generation and sequencing of the pooled DNA-library was sequenced on Illumina® NextSeq500 platform according to the manufacturer’s instructions (Australian Genome Research Facility, Melbourne).

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