Raw data for "Letter to the Editor re “Characterization of alginate-brushite in-situ hydrogel composites” http://dx.doi.org/10.1016/j.msec.2016.08.074

Published: 07-09-2016| Version 1 | DOI: 10.17632/599tczpphw.1
Contributor:
Sindre H. Bjørnøy

Description

pH measurements performed as described in the letter. Raman spectra of 3 randomly chosen beads produced at pH 7 as described in the letter.

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Steps to reproduce

5 mL of 1 mass% sodium alginate solution (15–20 cP as purchased from Sigma Aldrich, Oslo, Norway) containing 0.1 M (NH4)2HPO4 (Sigma Aldrich) into 50 mL of 0.25 M Ca(NO3)2 (Sigma Aldrich) solution, both adjusted to pH 7 or 8, at a rate of 5 mL/h. Raman analysis were performed on beads made at pH 7 after 24 h storage in the gelling solution. The beads were washed with DIW prior to analysis to wash away any residual ions (especially nitrate yields a strong Raman signal). A 532 nm laser was used at 100 % power with 100 accumulations of 1 s exposure.