Molecular data for: First report of glyphosate-resistant biotype of Eleusine indica in Europe
Eleusine indica is a common weed that infests crops, turfs, and orchards. Chemical control of this weed is often based on glyphosate, an herbicide that inhibits the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). When the same herbicide is repeatedly used over years, target species might evolve resistance mechanisms that allow them to avoid the herbicide activity. A glyphosate-resistant biotype (19-1) of Eleusine indica has been recently found in Italy and ten plants that survived to glyphosate application (360 g ae ha-1) were collected to sequence the EPSPS gene and look for eventual point mutations. Primers EI_EPSP_F2 (5' TCTCAGGGAACAACTGTGGTG 3') and EI_EPSP_R2 (5' GGCAGTCAGTGCCAAGGAAA 3') were used to amplify a sequence of 460 bp, spanning the EPSPS codons 102 and 106. Point mutations at these positions are indeed widely known to endow glyphosate resistance in many species. The primer EI_EPSP_F2 was used for sequencing. All the 10 plants had the point mutation proline to alanine at position 106 (P106A, CCA to GCA) and therefore the main resistance mechanism was target-site mediated. This repository stores the Sanger Sequencing data of these 10 sequences plus 4 of susceptible plants (wild-type Eleusine indica EPSPS, biotype 19-S).