DBiT-seq sequencing data for mouse embryo

Published: 17-09-2020| Version 1 | DOI: 10.17632/5d7pvrb3pp.1
Yang Liu,
Mingyu Yang,
Yanxiang Deng,
Rong Fan


Title: High spatial resolution multi-omics atlas sequencing of mouse embryos Organism: Mus musculus Experiment type: Expression profiling by high throughput sequencing Summary: Microfluidic deterministic barcoding of mRNAs and proteins in tissue slides followed by high throughput sequencing enables the construction of high-spatial-resolution multi-omics atlas at the genome scale. Applying it to mouse embryo tissues revealed major tissue (sub)types in early-stage organogenesis, brain micro-vasculatures, and the fine structure of an optical vesicle at the single-cell-layer resolution. Overall design: Applying new approach – microfluidic Deterministic Barcoding in Tissue for spatial omics sequencing to mouse embryo tissues in different resolution (10μm, 25μm, 50μm).