DBiT-seq sequencing data for mouse embryo

Published: 17 September 2020| Version 1 | DOI: 10.17632/5d7pvrb3pp.1
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Description

Title: High spatial resolution multi-omics atlas sequencing of mouse embryos Organism: Mus musculus Experiment type: Expression profiling by high throughput sequencing Summary: Microfluidic deterministic barcoding of mRNAs and proteins in tissue slides followed by high throughput sequencing enables the construction of high-spatial-resolution multi-omics atlas at the genome scale. Applying it to mouse embryo tissues revealed major tissue (sub)types in early-stage organogenesis, brain micro-vasculatures, and the fine structure of an optical vesicle at the single-cell-layer resolution. Overall design: Applying new approach – microfluidic Deterministic Barcoding in Tissue for spatial omics sequencing to mouse embryo tissues in different resolution (10μm, 25μm, 50μm).

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Institutions

Yale University

Categories

RNA Sequencing, Protein Sequencing

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