Sequential enhancer sequestration dysregulates recombination center formation at the IgH locus

Published: 11 February 2018| Version 1 | DOI: 10.17632/5f23hhtpnj.1
Xiang Qiu


Here we report that in the absence of intergenic control region 1 (IGCR1) the intronic enhancer (Eµ) associates with the next available CTCF binding site located close to VH81X, via putative heterotypic interactions involving YY1 and CTCF. Figure 1 and S3 show that Eµ loops to an alternate site close to the 3’-most functional VH gene segment, VH81X, upon disruption of its interaction with IGCR1. Figure 2 and S4 support that Eu-VH81X loop requires both YY1 and CTCF. Figure 3 and S5 demonstrate that DFL16.1 utilization is reduced on IGCR1-deficient alleles. Figure 4 and S6 shown that VH (VH713 and VHQ52) recombination is greatly induced and Eu-dependent on IGCR1-deficient alleles. Figure S7 show the verification of IGCR1 deleted cell line. Figure 6 and S8 support that distal VH is replaced from recombination center. Figure S10A show the versification of proximal VH deletion. Figure S10B provide information for recombination in different cell lines.



National Institute on Aging Intramural Research Program


Immunology, Chromatin