Raw data for: Comparative analysis of oil palm extraction rates and nutritional profiles of indigenous and introduced hybrid genotypes cultivated in selected districts of Uganda.

Description

This dataset contains raw measurements and analytical results from ripe oil palm fruit bunches sampled in three Ugandan districts: Kalangala (introduced hybrids), Bundibugyo, and Kanungu (indigenous genotypes). A total of 75 bunches were collected, georeferenced, and processed for oil extraction and compositional analysis. The raw data include detailed field and sample metadata such as genotype, cultivation scale, plant age, agronomic practices, and morphological traits, alongside oil extraction parameters including oil extraction rate and mass of stearin recovered. In addition, the dataset provides biochemical analyses covering carbohydrate content determined by the Anthrone method, fatty acid methyl ester (FAME) profiles for linolenic, linoleic, palmitic, stearic, and oleic acids using HPLC-UV, and beta-carotene concentration measured spectrophotometrically. All assays were performed in triplicate with analytical-grade reagents, and results are expressed in standardized units (mg/100 g, μg/g). Statistical outputs include raw values and processed data from non-parametric Kruskal-Wallis tests, comparing genotypes, districts, and age categories. Together, these data offer a comprehensive resource for evaluating oil palm diversity, nutritional composition, and biochemical quality across introduced and indigenous genotypes in Uganda.

Steps to reproduce

Study Sites & Sampling • Select three districts: Kalangala (introduced hybrids), Bundibugyo, and Kanungu (indigenous genotypes). • Identify ripe bunches using Malaysian Palm Oil Board standards (10–50% fruits naturally detached). • Collect 75 ripe bunches (33 Kalangala, 20 Bundibugyo, 16 Kanungu). • Georeference sampled trees with GPS, label, weigh, and transport bunches to NaCRRI laboratory. Oil Extraction • Detach fruits, wash, and separate mesocarp. • Dry mesocarp in solar dryer (24 h). • Press 50 g samples using electric screw press (40–100 °C). • Centrifuge extracts at 55,865 g for 25 min at 30 °C. • Measure olein and stearin; calculate oil extraction rate (OER) and mass of stearin recovered (MOSR). • Carbohydrate Analysis (Anthrone Method) • Mix palm olein with ethanol, incubate (70 °C, 2 h), cool (4 °C), centrifuge. • Add sulfuric acid, incubate (99 °C, 7 min). • Measure absorbance at 620 nm; calculate carbohydrate concentration using calibration formula. • Perform triplicates per sample. • Fatty Acid Methyl Ester (FAME) Analysis • Saponify 0.5 g oil with methanolic KOH. • Wash with ultrapure water, extract with dichloromethane. • Dry over magnesium sulphate, filter, evaporate volatiles. • Analyze with HPLC-UV (C18 column, 205 nm detection, 100% acetonitrile mobile phase). • Identify and quantify linolenic, linoleic, palmitic, stearic, and oleic acids using standards. • Beta-Carotene Determination • Extract olein with hexane (95%). • Measure absorbance at 449 nm. • Calculate concentration using Beer-Lambert law (μg/g basis). Data Analysis • Record raw data in Excel; import into SPSS v26. • Remove outliers (e.g., KU17, KL34–KL38). • Test normality (Shapiro-Wilk) and homogeneity (Levene’s). • Apply Kruskal-Wallis H test for group comparisons (genotypes, districts, age categories). • Determine significance at p < 0.05. • Generate graphs in Excel.

Institutions

• Muni University

  Northern Region, Arua

Categories

Funders

• IFAD

  Grant ID: NOPP2020

Licence