Action spectrum of redox state of plastoquinone pool

Published: 11 June 2020| Version 1 | DOI: 10.17632/5grmz2gv8b.1
Contributors:
Esa Tyystjarvi,
Heta Mattila,
Sergey Khorobrykh,
Marja Hakala-Yatkin,
Vesa Havurinne,
Iiris Kuusisto,
Taras Antal,
Taina Tyystjärvi

Description

The files contain the primary data for measurements regarding the effects of visible wavelengths on the redox state of the plastoquinone pool of plant chloroplasts. The data include Absorptance spectrum of Arabidopsis thaliana leaves Action spectrum of photosynthetic electron transfer rate (ETR) in Arabidopsis thaliana Action spectrum of Fo fluorescence yield in Arabidopsis thaliana Action spectrum of plastoquinone redox state in Arabidopsis thaliana leaves Amount of plastoquinone in Arabidopsis leaves Excitation spectrum of F735/F685 ratio of Arabidopsis thylakoids Dependence of light state on an illumination treatment of Arabidopsis leaf and on the wavelength used as actinic light in 77 K fluorescence measurement Demonstration of fluctuation of redox state of plastoquinone pool during illumination of Arabidopsis leaves with fluctuating light Measurements of postillumination Fo rise, including - Data showing that Fo rise is variable fluorescence - Action spectrum of Fo rise Determination of the redox state of the plastoquinone pool after extraction of plastoquinone with a transparent mortar before HPLC analysis Measurements of redox state of plastoquinone pool after illumination of Arabidopsis leaves with white light through thin leaves of Aponogeton ulvaceus OJIP measurements from Arabidopsis leaves in the absence and presence of DCMU Dependence of the relative magnitude of the P700+ signal on wavelength of illumination of Arabidopsis leaves Dependence of plastocyanin redox changes on the wavelength of illumination Western blots with phosphothreonin antibody showing LHCII phosphorylation at different wavelengths of visible light Measurements of the redox state of the plastoquinone pool at different intensities of natural sunlight and at different intensities of 440 nm PSI light and 470 nm PSII light Data from measurements of the plastoquinone pool size of Arabidopsis Action spectrum of the redox state of the plastoquinone pool in Arabidopsis, measured with HPLC after illumination of leves at selected wavelegths (PPFD 50 umol/m2s, 4 min). These data also include the original HPLC chromatograms. Redox state of plastoquinone pool after illumination with several different polychromatic lamps Quantification of (oxidized) plastoquinone remaining after reduction of the HPLC sample with NaBH4 Modeling of plastoquinone redox state in polychromatic light. The data also contain the modeling software with source code. Spectra of the lamps used in the study, and sunlight in the location and time of the measurements done in natural sunlight Software used for calculation of the redox state of the plastoquinone pool from the spectrum of polychromatic light State transition measurements with 77 K fluorescence spectroscopy after 1 h illumination of Arabidopsis leaves at wavelengths affecting the redox state of the plastoquinone pool

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Plant Biology

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