Nitrogen cycling functional gene abundance and potential activity in novel perennial forage cropping systems in Rwanda

Published: 26 January 2023| Version 1 | DOI: 10.17632/5h66s39r5j.1
Contributor:
Marie Schaedel

Description

This data is associated with a study entitled "Nitrogen cycling functional gene abundance and potential activity in novel perennial forage cropping systems in Rwanda", in which we sampled bulk soil from two locations in Rwanda cross a dry to rainy season transition. We evaluated the degree to which legume intercropping affected soil nitrogen status and putative biological nitrification inhibition in the companion intercrop species. There are two data sets associated with this study: 1. The file entitle "soil_data.csv" contains all measurements related to bulk soil samples (n=335). We measured nitrification potential (NP), denitrification enzyme activity (log_dea) that was log-transformed for analysis, permanganate-oxidizable carbon (mg_kg_POXC), nitrate (mg_kg_NO3N), ammonium (mg_kg_NH4N), potentially mineralizable nitrogen (net_NH4N), pH, gravimetric water content (gwc), total extractable nitrogen (TN), and non-purgeable organic carbon (NPOC). In addition, this file contains metadata such as sampling location, plant treatment, block, and collection timepoint. 2. The file entitle "NiCE_data.csv " contains data from a high-throughput qPCR experiment in which we quantified twenty nitrogen cycle functional soil from the bulk soil samples from the previous dataset. This file contains: a qPCR assay number, the assay target gene, forward and reverse primers, soil ID number, gene concentration, Ct value, and the log10-transformed gene copy number.

Files

Steps to reproduce

For information on experimental methods and statistical analysis, refer to the forthcoming manuscript.

Institutions

University of Minnesota

Categories

Applied Microbiology, Soil, Nitrogen, Carbon, Agricultural Soil, Soil Fertility, Nitrogen Cycle, Tropical Crops

Funding

National Science Foundation

00074041

Consortium of International Agricultural Research Centers

University of Minnesota

Doctoral Dissertation Fellowship

Licence