Investigating the mechanism of action of aggregation-inducing antimicrobial Pept-ins
Raw NGS data, the data set is related to figure 3G-3I.
Steps to reproduce
A single colony isolated from MH plates was grown in 50 mL MH medium in the absence of antibiotics until the end-exponential growth phase. The genomic DNA of collected bacteria was extracted using MagAttract HMW DNA Kit (Qiagen). Genomic DNA samples were sent to MicrobesNG for downstream library preparation, sequencing and bioinformatic analyses (trimming of raw data, alignment to the reference genome and variant calling). For genome annotation as well as variant calling, genome sequence (AP012030) downloaded from NCBI for E. coli strain DH1 was used as reference. Mutations occurred in ancestor strains were removed from all samples before further analysis.