Impact of Surfactant protein B (SP-B) on high density lipoprotein (HDL) oxidation index
Circulating immature surfactant protein B (proSP-B) forms emerged as the most reliable lung-specific circulating marker for alveolar-capillary membrane (ACM) dysfunction and for the overall clinical status of heart failure. Of note, ProSP-B forms were detectable in high-density lipoprotein (HDL) only. Thus we assessed the impact of proSP-B on HDL functions using HDL from healthy subjects enriched with proSP-B produced by a stably transfected CHO cell line that specifically expresses and releases the human proSP-B. In particular, HDL antioxidant capacity (HOI) has been assessed in terms of ability to inhibit air-induced LDL oxidation. Data from this dataset demonstrated that HOI was impaired after proSP-B enrichment (Table1). HOI was also higher in HF patients with respect to age-matched control healthy subjects (Table2).
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HOI was measured using a Dichlorofluorescein (DCF)-based fluorescent assay, as described by Breton et al (doi: 10.1016/j.atherosclerosis.2013.10.034). Data are expressed as HDL oxidant index (HOI), calculated as the ratio of fluorescence in the presence of HDL divided by the fluorescence in the absence of HDL.