Datasets of "On the usefulness of mock genomes to define heterotic pools, testers, and hybrid predictions in orphan crops"

Published: 6 December 2022| Version 1 | DOI: 10.17632/5rtc89t7v5.1
Contributors:
Roberto Fritsche-Neto,
Ingrid Machado,
Julio DoVale,
Felipe Sabadin

Description

The advances in genomics in recent years have increased the accuracy and efficiency of breeding programs for many crops. Nevertheless, the adoption of genomic enhancement for several other crops essential in developing countries is still limited, especially for those that do not have a reference genome. These crops are more often called orphans. This is the first report to show how the results provided by different platforms, including the use of a simulated genome called the mock genome, can generate in population structure and genetic diversity studies, especially when the intention is to use this information to support the formation of heterotic groups, choice of testers and genomic prediction of single-crosses. For that, we used a method to assemble a reference genome to perform the SNP calling without needing an external genome. Thus, we compared the analysis results using the mock genome with the standard approaches (Array and GBS). The results showed that the GBS-Mock presented similar results to the standard methods of genetic diversity studies, division of heterotic groups, the definition of testers, and genomic prediction. These results showed that a mock genome constructed from the population's intrinsic polymorphisms to perform the SNP calling is an effective alternative for conducting genomic studies of this nature in orphan crops, especially those that do not have a reference genome.

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Institutions

Universidade Federal do Ceara, Universidade de Sao Paulo Escola Superior de Agricultura Luiz de Queiroz

Categories

Quantitative Genetics, Plant Breeding

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