Lipidomics analysis of primary human T cells after LXR activation with GW3965
Description
The liver X receptors (LXR) are important transcriptional regulators of cholesterol, fatty acid, and phospholipid metabolism. Here we explored the effect of LXR activation on the lipid content of primary human T cells. The dataset provided contains pmol concentrations of 701 lipid species in T cells treated with a synthetic LXR agonist (GW3965) for 36 hours. Additional tabs contain the processed data which was used to generate the figures and tables presented in the referenced publication.
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Steps to reproduce
CD3+ T cells from 4 healthy donors were sorted by FACS and plated at (n=4). were treated with DMSO (CTRL) or GW3965 (GW, 1 µM) for 36 hours. A total of 10-15 million cells were incubated in 12-well plates at a density of 5 x 10^6/mL in RPMI 1640 supplemented with 10% foetal bovine serum and pen-strep. At the end of the culture, cells were washed twice in PBS and frozen cell pellets were shipped to Lipotype GmbH (Dresden, Germany) for mass spectrometry-based lipid analysis. For statistical analysis lipid species with less than n=3/condition were excluded. Lipid concentrations were converted to percentage of total lipid per sample (molpercent). Lipid concentrations in control and GW3965 treated samples were compared using a paired two-tailed t-test to account for baseline variation between human donors.