Characterization and function of progesterone receptor membrane component 2 (PGRMC2) in the human endometrium - Annex 1.
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Supplementary material of the Ph.D. thesis entitled “Characterization and function of progesterone receptor membrane component 2 (PGRMC2) in the human endometrium”, presented by Yassmin Medina Laver, under the direction of Dr. Francisco Domínguez Hernández and Dr. José Bellver Pradas.
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PGRMC2’s role during decidualization was further examined through transcriptomic (performing RNA-seq technique) and proteomic (using SWATH-MS procedure) analyses following siRNA-mediated PGRMC2 silencing. The silencing of PGRMC2 in d-hEnSCs led to the identification of 4687 differentially expressed genes (DEGs) and 28 differentially expressed proteins (DEPs) in d-hEnSCs. Genes were considered relevant when adjusted P < 0.01 and fold change was greater than 2 or less than 0.5. Functional enrichment analysis of the DEGs revealed that the 2420 upregulated genes after PGRMC2 silencing were primarily associated with processes such as endoplasmic reticulum function, vesicular transport, cell morphogenesis, angiogenesis, cell migration and cell adhesion. In contrast, the 2267 downregulated genes were linked to key biological pathways, including aerobic respiration and protein biosynthesis. At the proteomic level, enrichment analysis of the altered proteins highlighted their roles in aerobic respiration, cellular responses, metabolism, localization of endoplasmic reticulum proteins, and ribonucleoside biosynthesis.
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Generalitat Valenciana
ACIF/2019/262