Cisplatin Damaged Gene Loci
Cisplatin is a classical example of a DNA-targeted genotoxic anticancer drug, yet the exact gene loci attacked by cisplatin as well as the relationship between these gene lesions and action of the drug remain largely unexplored. In this work, we have used a forward chemical genetics method to localize and identify cisplatin damaged gene loci on the whole human genome. By combining affinity separation, high throughput sequencing and mapping to human genome (hg38), we have mapped 14012 protein-encoding genes containing platination lesions, of which 445 genes belong to 628 known gene targets for cancer therapy reported previously. Significantly, cisplatin induces lesions on 306 protein kinase genes with high fold-enrichment during our gene loci mapping, accounting for a remarkable 59% of putative human protein kinase genes. The cisplatin-damaged protein kinase genes are involved in 260 highly associated core-signaling pathways. The data deposited into this database are the mapping results of cisplatin damaged DNA for two biological replicates.