PAI-1-dependent inactivation of SMAD4-modulated junction and adhesion complex in obese endometrial cancer. Lin et al.
Figure 1C. Representative sections of tissue microarrays immunofluorescently stained with the white adipose tissue 7 (WAT7) homing peptide, a known marker specific for ASCs (pink-red; see white arrows), CD31 for endothelial cells (green), and DAPI for nuclei (blue). Figure 4B-C. PLA indicating protein-protein interactions of PAI-1 and low-density lipoprotein receptor-related protein 1 (LRP1) on the cell surface (B) or intracellular (C). Figure 4F.PLA demonstrating protein-protein interactions of SMAD4 and ubiquitin due to ASC-CM exposure in the presence or absence of the following inhibitors: tiplaxtinin (Tip) for PAI-1, RAP for LRP1, and protease inhibitor MG132. Micrograph images showing positive PLA signal (green) for SMAD4-ubiquitin interactions. DAPI was used to stain nuclei (blue).