Sex- and gamete-specific patterns of X chromosome segregation in a trioecious nematode. Tandonnet et al.
This excel document contains information for the genotyping of 5 X chromosome markers and 5 autosomal (LG4) markers of the nematode Auanema rhodensis. Each marker contains a Single Nucleotide Polymorphism (SNP) between two strains of A. rhodensis (APS4 and APS6). This SNP creates a restriction enzyme cut site in one strain but not the other. Genotyping of the markers is performed by PCR amplification followed by digestion with a restriction enzyme. This spread sheet includes, for each marker, the full marker sequence, the position and nucleotide difference characterizing the SNP, the forward and reverse primers used to amplify the marker, the restriction enzyme used to differentiate one strain from the other, the strain being cut and the undigested and digested PCR product sizes.