Raw mass spectrometry data of recombinant human a1B/b3-tubulin
Published: 11 July 2019| Version 1 | DOI: 10.17632/64yp27r788.1
Recombinant human a1B/b3-tubulin was purified from High Five insect cells. We run about 5 μg of protein on a 4-20% Tris-Glycine SDS-PAGE gel. The tubulin band is excised, reduced, alkylated, trypsinized, and analyzed by tandem LC-MS/MS.