Raw mass spectrometry data of recombinant human a1B/b3-tubulin

Published: 11-07-2019| Version 1 | DOI: 10.17632/64yp27r788.1
Contributor:
Shih-Chieh Ti

Description

Recombinant human a1B/b3-tubulin was purified from High Five insect cells. We run about 5 μg of protein on a 4-20% Tris-Glycine SDS-PAGE gel. The tubulin band is excised, reduced, alkylated, trypsinized, and analyzed by tandem LC-MS/MS.

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