Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators
Ferroptosis, an iron-dependent programmed cell death triggered by excessive lipid peroxidation, has shown promising therapeutic potentials in human diseases. Here, we describe a protocol of a CRISPR-Cas9 loss-of-function screen to identify regulators in response to different inducers of ferroptosis. We emphasize the steps of library amplification, drug treatment, high-throughput sequencing preparation, and bioinformatics analysis using Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout (MAGeCK). We also present a method to discover the regulators of ferroptosis and verify the potential targets efficiently.