Supplemental video 6.C. BCG-hMCs interactions are increased by IL-33 pre-treatment

Published: 4 January 2021| Version 1 | DOI: 10.17632/679j32jt45.1


hMCs were treated for 24h with IL-33 (50ng/ml) or left unstimulated (control). After incubation cells were washed and left 30min in an 8 well chamber at 37C. BCG mCherry at a MOI 10:1 was added to wells and a video was recorded for 8h using confocal microscopy at 37C and 5% CO2 with 30-second interval acquisitions. Videos were analysed using FIJI and compressed to 4 minutes to allow visualization. Video 1 shows naïve MCs and Video 2 shows IL-33 pre-treated MCs. Videos are representative of 2 independent experiments

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The University of Manchester


Immunology, Microbiology