Single cell antibody sequences from ApoE mouse aortic germinal center B cells

Published: 18 April 2024| Version 1 | DOI: 10.17632/67gyxw9j7n.1
Contributors:
Robert Maul,
,
,
,

Description

Immunoglobulin Heavy and Light chain sequences from germinal center B cells derived from the ApoE-/- mouse aorta. Mice were place on a high-fat diet at 12 weeks of age and germinal center B cells were collect at 28 weeks of age. Samples were single cell sorted, reverse transcribed using a template switch oligo, and PCR amplified from the IgH or IgK constant region to the variable region leader sequence. Files are provided as FASTA files.

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Institutions

National Institute on Aging

Categories

Atherosclerosis, Antibody, B Cell

Funding

National Institute on Aging

Licence