Single cell antibody sequences from ApoE mouse aortic germinal center B cells

Description

Immunoglobulin Heavy and Light chain sequences from germinal center B cells derived from the ApoE-/- mouse aorta. Mice were place on a high-fat diet at 12 weeks of age and germinal center B cells were collect at 28 weeks of age. Samples were single cell sorted, reverse transcribed using a template switch oligo, and PCR amplified from the IgH or IgK constant region to the variable region leader sequence. Files are provided as FASTA files.

Institutions

• National Institute on Aging

  MD, Bethesda

Categories

Funders

• National Institute on Aging

  Maryland, United States

Licence