Mechanical stimulation from the surrounding tissue activates mitochondrial energy metabolism in differentiating germ cells. Wang et al., 2023
Description
The data file contains 16 original data folders, with microscopy images and GraphPad files for quantifications. Figure 1. Cyst flattening drives JNK-mediated OXPHOS activation during differentiation. Figure 2. Membranes at the germ cell interfaces are stretched in the flattened differentiating cysts. Figure 3. OXPHOS activation in differentiating cysts requires increased membrane stretching. Figure 4. Tmc is required for OXPHOS activation in differentiating cysts. Figure 5. Tmc maintains cytosolic Ca2+ concentration in differentiating cysts. Figure 6. Cytosolic Ca2+ triggers OXPHOS activation in differentiating cells through CaMKI and Fray. Figure 7. A schematic of the mechanotransduction pathway that activates OXPHOS during ovarian cyst differentiation. Supplemental Figure S1. pFCs’ wrapping is required for flattening and OXPHOS induction of differentiating cysts, related to Figure 1. Supplemental Figure S2. Flattening of differentiating cysts induces the IIS-Myc-ETC biogenesis axis, related to Figure 1. Supplemental Figure S3. Membranes at the germ cell interfaces are stretched in flattened differentiating cysts, related to Figure 2. Supplemental Figure S4. Regulation of plasma membrane stretching in differentiating cysts by manipulating cortical tension, related to Figure 3. Supplemental Figure S5. OXPHOS induction in differentiating cysts requires Tmc, related to Figure 4. Supplemental Figure S6. Calibration of the UASz-mTagBFP2-P2A-GCaMP6s fusion protein in S2 cells and cytosolic Ca2+ properties in undifferentiated and differentiating germline cysts, related to Figure 5. Supplemental Figure S7. Expression of Tmc and JNK mRNA in germaria and the specificity of DNA probes used in smFISH, related to Figure 5. Supplemental Figure S8. Germline expression of PV impairs JNK and OXPHOS induction in differentiating cysts, related to Figure 5. Supplemental Figure S9. Mitochondrial Ca2+ is essential for OXPHOS induction in differentiating cysts, related to Figure 5. Supplemental Figure S10. Fray is downstream of CaMKI, related to Figure 6.