Maternal and neonatal microbiome diversity and metabolic pathways are modified by a gastrointestinal nematode in pregnant and lactating mice
Published: 11 August 2020| Version 2 | DOI: 10.17632/6dygr4kdz8.2
Contributors:
Manjurul Haque, Kris Koski, Description
Pregnant mice were infected with Heligmosomoides bakeri nematode on gestation day 7, 12 and 17 and on postnatal day 3. Vaginal lavage was collected from dam on post-natal day 2. Pup stomach content was collected on postnatal day 2 and 7. Dam intestinal content was collected on post-natal day 2 and day 9 and dam milk was collected on postnatal day 9. After DNA isolation the samples were subjected to Miseq sequencing of bacterial 16S rRNA. Data was analysed using QIIMME2
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Institutions
McGill University
Categories
Metagenomics