Kocuria rhizophila and Micrococcus luteus as an emerging opportunist pathogens for cultured salmonids.

Published: 4 January 2018| Version 1 | DOI: 10.17632/6hdwwspjnb.1
Contributors:
Agnieszka Pekala,
Jerzy Antychowicz,
Ewa Pazdzior,
Wiktor Niemczuk,
Beata Wiecek,
Alicja Bernad,
Hanna Glowacka

Description

In 2014 and 2016 in Poland a few disease outbreaks caused by Kocuria rhizophila and Micrococcus luteus were diagnosed in rainbow trout and brown trout. In each of these events, abnormal mortality (approximately 50%) was accompanied by pathological changes in external tissues and internal organs. Exophthalmia, swollen abdomen, increased enhanced skin melanisation as well as skin petechiaes and focal lesions were often seen in moribund fishes. In many fish inflammation of the intestine, liver congestion, and hemorrhages in the tail part of the muscles, were also observed. Samples from moribund fish, internal organs were inoculated onto blood agar and trypticase soya agar, and incubated for up to 3-4 days at a temperature of 27°C. In the majority of cases uniform growth of Kocuria rhizophila or Microccocus luteus colonies were observed; and sometimes these bacteria appeared in predominant numbers. The bacteria identifications were performed using standard kits (API 20 Staph and Vitek 2). In order to improve the biochemical identification of isolated bacteria, for establishing the evolutionary relationships of their bacteria’s taxa, and finding the possible source of the fish infection, sequencing was applied. Comparison of our strainsˊ molecular structures with the available data in GenBank showed that Kocuria rhizophila or Micrococcus luteus had never been isolated from diseased fish before, and that our isolates were very similar to strains which had been isolated from food processing environments (in the case of Kocuria rhizophila) and from scallops (Micrococcus luteus). The challenge tests performed with our strains of Kocuria and Micrococcus on rainbow trout in laboratory aquaria confirmed the three Koch postulates. Antibacterial disc diffusion studies using disc - diffusion methods showed that Kocuria rhizophila and Micrococcus luteus are sensitive to most of the drugs tested i.e. tetracycilnes, β-lactams, macrolide, aminoglycoside, and also amphenicols. The results of these study show that control of outbreaks of the diseases in rainbow trouts or brown trouts seems realistic if caused eventually by Kocuria rhisophila or Micrococcus luteus.

Files