Oxygenated Water could augment Doxorubicin to act against Diethylnitrosamine induced Hepatocellular Carcinoma in Rats

Published: 21 February 2024| Version 1 | DOI: 10.17632/6k52mgff5c.1
Mohamed Elborei


The present study was undertaken to evaluate the possible synergistic effect of oxygenated water with doxorubicin against diethylnitrosamine-induced hepatocellular carcinoma in rats. Hepatocellular carcinoma was induced by diethylnitrosamine added to the drinking water at a dose of (100 mg/L) for 8 consecutive weeks followed by 8 weeks of drinking tap water. Diethylnitrosamine administration caused elevation in serum liver enzymes, serum tumor markers, caspase-3 content and depletion of the antioxidant enzymes in the liver tissue. This was confirmed by histopathological investigations. Interestingly, treatment with oxygenated water (5 ml/kg/day, p.o) concurrently with intravenous doxorubicin administration via tail vein (2.5 mg/kg/week) for 4 weeks starting from the 13 th week corrected serum liver enzymes, caused significant decrease in serum tumor markers, increased the hepatic tissue content of caspase-3, restored the depleted antioxidant enzymes and corrected the histopathological finding. In conclusion, oxygenated water enhanced the doxorubicin efficacy, most likely due to the anti-hypoxic effect.



Pharmacology, Oncology