miR-204 regulates SIRT1-mediated changes associated with metabolic memory induced by high glucose in hRPE cells
These datasets mainly involve cell experiments of diabetic retinopathy, and discuss the relationship between metabolism memory induced by high-glucose and related pathways through basic research methods.
Steps to reproduce
Molecular biology experiment. 1. Human retinal pigment epithelial cell culture before establishing high-glucose and metabolic memory model, followed by miR-204 plasimd infection. 2. Detecting related protein and mRNA by RT-qPCR, western blot and cellular immunohistochemistry staining, and detecting the apoptosis by flow cytometry. 3. Collecting data through ABI 7500, Bio-Rad, fluorescence microscope and BD AccuriC6. 4. Analysis of data using GraphPad Prism 6.0, Image J and SPSS.24