Datasets of "Immune priming depends on age, sex and Wolbachia in the interaction between Armadillidium vulgare and Salmonella"
In this study, we tested whether age, gender and the presence of the symbiotic bacterium Wolbachia affect the protection resulting from immune priming in A. vulgare against S. enterica. We firstly primed young and old, symbiotic and asymbiotic males and females, either with a non-lethal low dose of S. enterica, LB broth or without injection (control). 7 days post-injection, we performed a LD50 injection of S. enterica in all individuals and we monitored their survival rates. We demonstrated that survival capacities (Data_survival.csv) depend on age, gender and Wolbachia: young and old asymbiotic individuals both expressed immune priming (S. enterica-primed individuals survived better than LB-primed and non-primed), with a general decline in the strength of protection in old females, but not in old males, compared to young. When Wolbachia is present, the immune priming protection was observed in old, but not in young symbiotic individuals. We also quantified the load of Wolbachia in order to test whether this difference between young and old symbiotic individuals could be explain by the symbiont load (Data_Wolbachia.csv). No difference was observed between these two groups. The R_script file contain all the statistical analyses performed on datasets giving these results.
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Detailed methods to obtain data are stated in the material and methods and supplementary material of the related research article. Statisticals analysis are given in the R_script file.