RNA-seq OE19 complex individual knockdowns
siRNA knockdowns of 5FMC complex members (PELP1, TEX10, LAS1L, SENP3, WDR18) . Conditions - addition of siRNA at times 0, 48hr according to manufacturer instructions. Resulting knockdowns were verified by qPCR and western blots, knockdown at over 90% in all samples. Aim - to assess the effects of 5FMC complex knockdown on gene expression.