RNA-seq OE19 complex individual knockdowns

Published: 29-10-2019| Version 2 | DOI: 10.17632/6nfpv4rz3v.2
Contributor:
Rotem Salmi-Leshem

Description

siRNA knockdowns of 5FMC complex members (PELP1, TEX10, LAS1L, SENP3, WDR18) . Conditions - addition of siRNA at times 0, 48hr according to manufacturer instructions. Resulting knockdowns were verified by qPCR and western blots, knockdown at over 90% in all samples. Aim - to assess the effects of 5FMC complex knockdown on gene expression.

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