Robust Automation and Point of Care IDentification of COVID
Description
This study aimed to validate the EasyNAT SARS-CoV-2-Assay, a diagnostic kit based on RT-LAMP, using oral and nasopharyngeal samples. The EasyNAT kit was compared to the Xpert Xpress SARS-CoV-2 kit, evaluating 873 samples obtained during routine analysis at the Microbiology Laboratory of the Hospital Costa del Sol (Marbella, Spain).
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Steps to reproduce
Samples were processed using the EasyNAT SARS-CoV-2 diagnostic kit and analyzer (Model: UC0104; Ustar Biotechnologies, Hangzhou Ltd.). The only manipulation before inserting the cartridge into the analyzer was dispensing the extraction solution, followed by adding 500μl of nasopharyngeal swab sample. This real-time RT-PCR kit can separately detect the ORF1ab and N SARS-CoV-2 target RNA, and includes two internal controls, employing cross-priming amplification (CPA) to detect human glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-mRNA, thus allowing monitoring the effectiveness of extraction, purification and amplification.
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Funding
Innovative Medicines Initiative
101005144