Proteomics raw data of the secretome of mesenchymal stromal cells from feline adipose tissue

Published: 1 October 2020| Version 1 | DOI: 10.17632/6r7kr2b3hc.1
Contributors:
Maria Laura Lara Arias,
Fabiana Souza,
rubia schmith,
Viviane Codognoto,
Bruna De Vita ,
Camila P Freitas-Dell’Aqua,
Fernanda C Landim,
Marina Landim-Alvarenga

Description

This data are related with a study to describe the secretome of mesenchymal stromal cells derived from adipose tissue (AD-MSCs) of the cats and to evaluate the effects of culture conditions on conditioned medium proteomics by AD-MSCs. We used samples of adipose tissue collected from 5 queens. The samples were prepared to culture and assayed for osteogenic and adipogenic differentiation, and immunophenotypic analysis. Four culture conditions were tested, normoxia with fetal bovine serum (N + FBS - control), hypoxia with FBS (H + FBS), normoxia without FBS (N – FBS) and hypoxia without FBS (H – FBS). The secretome was collected and prepared to proteomics (ESI Q-TOF MS/MS).

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The results were analyzed by univariate and multivariate analyses (MetaboAnalyst software - https://www.metaboanalyst.ca/). UniprotKB (www.UniProt.org.br) and PANTHER classification system (www.pantherdb.org) databases was used to analyze gene ontology terms. Venn diagram was obtained in the web tool from Bioinformatics & Systems Biology. Exponentially modified protein abundance index (emPAI) obtained by Mascot Distiller software was used to calculated the principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), which was used to indicate the importance of the proteins by average variable importance in the projection (VIP score, α ≥ 1). To this analysis, the results were tested by univariate and multivariate analyses (MetaboAnalyst software - https://www.metaboanalyst.ca/). A heatmap was also used to compare the protein abundance between the groups.