PROTECTION OF COENZYME Q-10 AGAINST CONTRAST-INDUCED ACUTE KIDNEY INJURY IN DIABETIC RATS
ABSTRACT Background: Diabetes Mellitus (DM) is an important risk factor for Contrast- induced acute kidney injury (CI-AKI). DM and CI-AKI results in oxidative damage and inflammation that can be reduce with treatment of coenzyme Q-10 (CoQ10). The aim of this study was to investigate the therapeutic potential of CoQ10 in renal function, renal hemodynamics, oxidative profile and renal histology in diabetic rats submitted to the CI-AKI model. Methods: Wistar rats, male, randomized into four groups: Citrate- control animals, received citrate buffer (streptozotocin vehicle, 0.4 ml); Tween- control animals of CoQ10 treatment, received 1% Tween 80 (CoQ10 vehicle, 0.5 ml); DM- animals that received streptozotocin (60 mg/kg); DM+IC: DM animals, treated with iodinated contrast (IC, 6 ml/kg); DM+IC+CoQ10: DM animals treated with CoQ10 (10 mg/kg) and that received with IC (6 ml/kg). The protocols lasted 4 weeks. Were evaluated the renal function by inulin clearance and serum creatinine, renal hemodynamics by renal blood flow (RBF) and renal vascular resistance (RVR), markers of oxidative stress such as urinary peroxides and nitrate, lipid peroxidation, thiols in renal tissue and renal histological analysis. Results: DM animals showed reduced renal function which was reflected with an increase of serum creatinine and significant reduced of inulin clearance, as well as a reduction on RBF, increased RVR and redox imbalance with a higher urinary peroxides, nitrate lipid peroxidation levels and depletion of thiols in renal tissue. IC treatment exacerbated theses changes in DM + IC. CoQ10 administration ameliorates renal function, prevented hemodynamic changes, neutralize oxidative damage and progression of the histologic damage in the DM+IC+CoQ10 group. Conclusion: This study demonstrated a renoprotection of CoQ10 in an experimental model of risk factor of DM for CI-AKI. CoQ10 presented an antioxidant effect on the CI-AKI in diabetic rats, by improving function and renal hemodynamics, preserving morphology and reducing oxidative stress. KEYWORDS: Diabetes Mellitus; Nephrotoxicity; Iodinated Contrast; Coenzyme Q-10.