WXL-Research Data-BSZY

Published: 20 December 2023| Version 1 | DOI: 10.17632/6xnsbs5d4h.1
Contributor:
xiaolan wang

Description

Objective: Chinese herbal prescription, Bushen Zhuyun Decoction (BSZY), has shown promising effects on gynecological infertility, but the mechanism is still unclear. This study aimed to investigate the regulatory effects of BSZY on endometrial receptivity, which plays important role in embryo implantation, and its regulatory mechanisms associated with NF-κB/NLRP3 signaling pathway. Methods: SD rats at reproductive age with affected endometrial receptivity was established using mifepristone (RU486), the regulatory effects of BSZY on endometrial receptivity was evaluated by H&E, and changes in sex hormones by ELISA and Western blot. Moreover, human endometrial RL95-2 cells were treated with H2O2, and inflammatory cytokines in rats and RL95-2 cells were analyzed by ELISA. The activation of NF-κB/NLRP3 signaling pathway in RL95-2 cells were characterized using immunofluorescence and Western blot. Mitochondrial morphology and function in RL95-2 cells were observed by TEM and cell mitochondrial stress test. Results: BSZY was found to increase uterine endometrial thickness and attenuate histopathological changes. BSZY also regulated endometrial ER and PR, and endocrine disorders and inflammation in pregnant rats. In addition, BSZY showed strong anti-inflammatory and cytoprotective effects in vitro. BSZY inhibited the activation of NF-κB/NLRP3 signaling pathway in RL95-2 cells, manifested as the regulation of P-NF-κB/NF-κB, NLRP3 and Caspase1. BSZY was also found to improve mitochondrial morphology and function. Conclusion: BSZY can improve endometrial receptivity, potentially by improving mitochondrial morphology and function to inhibit the activation of NF-κB/NLRP3 signaling pathway in endometrial cells, thus alleviate inflammation to improve endometrial receptivity.

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Gynecology

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