Biological Innocuousness of a Device Developed to Select the Optimum Spermatozoa to be used in the Treatment of Infertility

Published: 23-06-2021| Version 1 | DOI: 10.17632/6ygpz8562x.1
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Description

Neutral red uptake dataset Experiment number: experiment replicates Treatment:  BLANK, culture medium for fibroblast growth incubated in a polyropilene tube suitable for cell culture MEM, culture medium for fibroblast growth HTF, culture medium for sperm incubation MEM (SSA), culture medium for fibroblast growth pre incubated for 24 hs with the SSA acrylic device HTF (SSA), culture medium for sperm incubation pre incubated for 24 hs with the SSA acrylic device; HTF (SSA+P), culture medium for sperm incubation with 10pM of progesterone pre incubated for 24 hs with the SSA acrylic device) Dilution (Treatment:PBS), indicates the proportion of the mix made with the treatment solution and PBS solution. Cells incubation time (hs), time at which the cells viability was evaluated. Absorbance, the mean value of 3 replicates of the same treatment given by the microplate reader on each experiment.  Cell viability (%), is the percentage of viability calculated by dividing the absorbance of the treatment solution (minus the absorbance of PBS (treatment:PBS = 0:100)) by the absorbance of the blank solution (minus the absorbance of PBS) per 100. MTT assay dataset Experiment number: experiment replicates Treatment:  BLANK, culture medium for fibroblast growth incubated in a polyropilene tube suitable for cell culture MEM, culture medium for fibroblast growth HTF, culture medium for sperm incubation MEM (SSA), culture medium for fibroblast growth pre incubated for 24 hs with the SSA acrylic device HTF (SSA), culture medium for sperm incubation pre incubated for 24 hs with the SSA acrylic device; HTF (SSA+P), culture medium for sperm incubation with 10pM of progesterone pre incubated for 24 hs with the SSA acrylic device) Dilution (Treatment:PBS), indicates the proportion of the mix made with the tratmente solution and PBS solution. Cells incubation time (hs), time at which the cells viability was evaluated. Absorbance, the mean OD value of 3 replicates of the same treatment given by the microplate reader on each experiment.  Cell viability (%), is the percentage of viability calculated by dividing the absorbance of the treatment solution (minus the absorbance of PBS (treatment:PBS = 0:100)) by the absorbance of the blank solution (minus the absorbance of PBS) per 100. Colony formation dataset Experiment number: experiment replicates Treatment:  MEM, culture medium for fibroblast growth HTF, culture medium for sperm incubation MEM (SSA), culture medium for fibroblast growth pre incubated for 24 hs with the SSA acrylic device HTF (SSA), culture medium for sperm incubation pre incubated for 24 hs with the SSA acrylic device; HTF (SSA+P), culture medium for sperm incubation with 10pM of progesterone pre incubated for 24 hs with the SSA acrylic device);  Cells incubation (days), time at which the cells colony number was evaluated. Number of colonies, evaluated under direct observation in the microscope

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