Raw dataset of seminal proteomic from four purebred dogs
Hypothesis: Does dogs exhibit different protein profile of seminal plasma and spermatozoa among breeds? What data shows: These data show the proteomic profile and its respectively gene ontology of seminal plasma and sperm cells of four purebred dogs (Golden Retriever n = 3, Bernese Mountain Dog n = 4, Great Dane n = 3, Maremmano-Abruzzese Sheepdog n = 3), with mean ages and standard deviation of 4,0 ± 1,0 years (Golden Retriever), 2,0 ± 1,0 years (Bernese Mountain Dog), 1,4 ± 0,5 years (Great Dane) and 4,0 ± 0,7 years (Maremmano-Abruzzese Sheepdog), kenneled at Sao Paulo State, Brazil. Besides How it was gathered: Entire second fraction and a portion of the third semen fraction were collected into a silicone funnel attached to a graduated plastic tube by manual stimulation of the penis in the presence of a teaser bitch, when possible. The semen was subjectively evaluated at the kennel, and only ejaculate within normal seminal parameters considered for dogs, according to Kustritz et al. (2007), were used in this study. Spermatozoa and seminal plasma were separated by centrifugation and prepared individually for proteomic analysis by ESI Q-Tof mass spectrometer. The gene ontology annotation of the proteins found within the samples was obtained using the UniprotKB website (www.uniprot.org), and considered the molecular function, biological process and cellular component categories. How the data can be interpreted: There are two folders dataset. The "Seminal plasma and sperm cell proteins" folder contain two folders, one with all seminal plasma proteins, and other folder with all sperm cell proteins, which have individual files named by breed for each dog (n=13). The “Gene ontology of seminal plasma and sperm cell proteins” contain three files: Table S1, Table S2, and Table S3. The file Table S1 contain all proteins found in seminal plasma of evaluated dogs and their respective gene ontology. The file Table S2 contain all proteins found in spermatozoa of all dogs evaluated and their respective gene ontology. The file Table S3 contain all common proteins found in seminal plasma and spermatozoa of evaluated dogs and their respective gene ontology. References: Kustritz R. The value of canine semen evaluation for practitioners. Theriogenology 2007;68(3):329-37.